25 % Salt Water Media
|
NaCl
|
250.0 g
|
|
CaCl2*6H2O
|
0.2 g
|
|
KCl
|
5.0 g
|
|
MgSO4*7H20
|
10.0 g
|
|
Yeast Extract
|
10.0 g
|
|
Tryptone
|
2.5 g
|
|
Agar
|
20.0 g
|
|
Distilled Water
|
add to make volume 1.0 L
|
Make 2 solutions- one with yeast, tryptone,
and agar; the other with salts.
Adjust pH of nutrient solution to 7.0.
Sterilize seperately.
Mix and dispense asceptically.
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Methods
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