Phase I.
The group
initially used this method to attempt to select for an extremely halophilic
organism.
1.) All necessary supplies were procured (ie- 25% salt media, salted herring).Phase II.2.) Sample from the inside and outside of the salted herring were both innoculated onto the 25% salt media.
3.) The plates were incubated at 50 degrees Celsius for 48 hours.
4.) Plates were restreaked to attempt to maintain culture and a Gram stain was performed to attempt to identify the cell by its morphology.
5.) Another sample from the salted herring was innoculated into 25% media and incubated at 37 degrees Celcius after inconclusive results were obtained at 50 degrees Celcius.
6.) A sample of ocean perch was then obtained. The inside and outside of this sample was then streaked onto the 25% salt concentration plates.
7.) After inconclusive results were yet again obtained, samples from the ocean perch and salted herring were then innoculated onto 20% salt concentration plates.
8.) After yet another scathing defeat, the group moved to Phase II.
1.) Procure all necessary supplies (ie- salt water media, sea bass, squid)2.) Innoculate samples onto the synthetic seawater media plates from the inside and outside of the squid and sea bass specimen.
3.) Incubate at 37 degrees Celsius for 2 days. Store the squid and sea bass specimen together in a shallow bath of 3% salt water.
4.) Restreak each lab period to maintain a pure culture.
5.) Determine the characteristics and identity of the sample through Gram stains, oxidase tests, catalase tests, O/F tests, motility stabs, wet mounts, and growth on T-soy media.