Synthetic Seawater Media
3% salt Concentration
|
NaCl
|
27.0 g
|
|
CaCl
|
0.3 g
|
|
KCl
|
0.6 g
|
|
MgSO4*7H20
|
7.0 g
|
|
Na-glycerophosphate
|
0.2 g
|
|
Tris Buffer
|
2.0 g
|
|
Monosodium
|
5.0 g
|
|
Glutamum
|
1.0 g
|
|
Vitamin B12
|
1.0 mg
|
|
Distilled Water
|
add to make volume 1.0 L
|
Adjust pH to 7.5.
Sterilize with HCl.
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Methods
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